Abstract 1199: Global proteomic evaluation of the relationship between Epstein-Barr virus (EBV) and c-myc deregulation in endemic versus sporadic Burkitt lymphoma (BL)

Endemic BL (eBL) is characteristically positive(100%) for EBV, contrasting with sporadic(sBL) BL(∼30% EBV+). eBL vs sBL have different breakpoint regions within c-myc. The different mechanisms of lymphomagenesis however, remain unknown. Global analysis of proteins expressed in the different subtypes may provide insights into biologic, pathogenetic, and molecular differences. Objectives: To compare the proteomic expression profile and signal transduction pathways of EBV+ eBL with EBV+/- sBL and EBV+/- normal B-cells. Normal B-cells (EBV+/-) were isolated from leukopacks obtained from the NY Blood Center using Magnetic Cell Sorting CD20 MicroBeads (Miltenyi biotec, CA). Whole cell lysates obtained from EBV+ eBL Raji, EBV+ sBL NC37, EBV- sBL Ramos, and EBV± B-cells were digested and labeled with iTRAQ reagents. The peptides were resolved by 2D-LC technique. MS/MS spectra were acquired using an Orbitrap XL Tandem Mass Spectrometer (ThermoFisher). MS/MS data was searched using X! Tandem TPP software against human IPI database (v3.50) appended with decoy sequences. iTRAQ ratios of proteins (ProteinProphet probability of >0.9) were normalized and differentially expressed proteins were selected for further analysis. Over 400 proteins were identified; 827 binary differential protein expressions were established with a False Discovery Rate (FDR) of