Color-Coded Imaging Of Vessel Anastomosis In Vivo Using Rfp And Cfp Transgenic Mice

Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA We previously report the development of a color-coded imaging model that can visualize the anastomosis between blood vessels of red fluorescent protein (RFP)-expressing vessels in Gelfoam® previously vascularized in RFP transgenic mice and blood vessels of nestin-driven green fluorescent protein (ND-GFP) mice that express GFP in nascent vessels (Anticancer Res. 33: 3041-3046, 2013). In the present study, fourteen days after transplantation into RFP transgenic nude mice, the Gelfoam® was removed and re-transplanted into the subcutis on the flank of cyan fluorescent protein (CFP) transgenic mice. Skin flaps were made and anastomosis between the CFP-expressing vessels and RFP blood vessels in the Gelfoam® was imaged 14 and 21 days after re-transplantation. The results presented in this and our previous report suggest a new paradigm of visualizing blood vessel anastomosis and its implications for tumor angiogenesis. Citation Format: Fuminari Uehara, Yasunori Tome, Hiroki Maehara, Kazuhiro Tanaka, Fuminori Kanaya, Shinji Miwa, Yukihiko Hiroshima, Shuya Yano, Mako Yamamoto, Yasunori Matsumoto, Robert M. Hoffman. Color-coded imaging of vessel anastomosis in vivo using RFP and CFP transgenic mice. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 13. doi:10.1158/1538-7445.AM2014-13