Reversibility Of Monocytic Differentiation Of Hl-60 Cells By 1 Alpha,25 Dihydroxyvitamin D-3

Although 1 alpha,25(OH)(2)D-3 induces HL-60 cells to differentiate into monocytes, whether or not these monocytes revert to native promyelocytes is not clear. To investigate this question, HL-60 cells were treated with 1 alpha 25(OH)(2)D-3, and divided into CD14-positive and -negative cells with a cell sorter. These two populations were cultured with or without 1 alpha,25(OH)(2)D-3. Sorted CD14-positive HL-60 cells, treated with 1 alpha,25(OH)(2)D-3 for 7 days, reverted to CD14-negative cells and promyelocyte-like cells if 1 alpha,25(OH)(2)D-3 was removed from the medium. We conclude that the 1 alpha,25(OH)(2)D-3-induced differentiation of HL-60 cells into monocytes is reversible, and continuous administration of 1 alpha,25(OH)(2)D-3 is required for the differentiation of HL-60 cells. (C) 1997 Elsevier Science Ltd. All rights reserved.