Molecular cloning and expression of rat betacellulin cDNA 1 1 The sequence of rat BTC cDNA is DNA Da
Biochimica et Biophysica Acta(2000)
摘要
The cDNA encoding an entire open reading frame of rat betacellulin has been cloned from rat kidney. Expression of this cDNA in COS7 cells showed a significant amount of mitogenic activity in the culture media. Western blotting of the cell lysates suggested that the membrane-anchored precursor was cleaved to release its ectodomain very efficiently.
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关键词
cell differentiation,open reading frame,molecular cloning
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