Electrochemical sensor with dry reagents implemented in lab-on-chip for single nucleotide polymorphism detection

We developed an electrochemical (EC) sensor having dry reagents to detect pyrophosphoric acid (PPi) produced as a by-product of a polymerasechain-reaction (PCR) amplicon for single nucleotide polymorphism (SNP) detection. The EC sensor is implementable in a lab-on-chip (LoC) system, and a sensor chip having side-wall electrical connections that enable electrical contacts from the top of the LoC has been developed. We also developed separated on-chip placement of dry reagents divided into three groups in a sensor cavity to suppress background current when there is no PPi. Using this chip, we successfully demonstrated SNP detection in the ABO gene from human blood samples, in combination with the allele-specific PCR amplification method using our developed LoC system. (C) 2014 The Japan Society of Applied Physics