Development of CYP11B1 and CYP11B2 assays utilizing homogenates of adrenal glands: Utility of monkey as a surrogate for human

•CYP11B kinetics and inhibition was assessed using homogenates of adrenal glands.•Similarity between monkey and human CYP11B was demonstrated.•Comparable kinetics was observed for CYP11B1- and CYP11B2-mediated reactions.•Inhibition of human and monkey CYP11B1 and CYP11B2 showed good correlations inhibitors.•Cynomolgus monkey CYP11B assays are suitable surrogates for the human enzymes.