Examinations of allergenic proteins coding genes of some domestic apple cultivars

In our research we studied the occurrence of the main apple allergen coding gene-families (Mal d 1, Mal d 2, Mal d 3, Mal d 4) in 16 different and most preferably consumed apple varieties. After the DNA isolation by Wizard method the simple PCR reaction was used to examine the apple allergen-coding genes. To identify the presence of the four allergenic protein-coding genes two primer pairs were chosen. The presence of these allergens in most apple varieties could be confirmed. According to our results two varieties - Jonathan and Granny Smith - were found to contain the lowest amount of the coding genes of the allergenic apple proteins studied by us. Besides this, polymorph pattern was obtained by the use of Mal d 1 primer, which may lead to determine apple varieties with small amount of Mal d 1 allergens. The confirmation study of the presence of potential apple allergens by RNA and protein techniques is our plan in the near future.