Development of a prelabeling approach for a targeted nanochelator

The polymer polyethyleneimine with primary and secondary chelators can retain 225 Ac and its daughters. In this study we optimize a prelabeling approach, followed by addition of secondary chelators and explore crosslinking approaches to add a targeting molecule. A (N -Succinimidyl 3-(2-pyridyldithio)-propionate crosslinking approach was used to obtain a ~1 to 1 ratio of the modified PEI to Trastuzumab. This approach coupled with the prelabeling approach would allow the synthesis of the radiolabeled targeted polymer for 225 Ac radioimmunotherapy.