Alpha-Cell Dysfunctions And Molecular Alterations In Male Insulinopenic Diabetic Mice Are Not Completely Corrected By Insulin
ENDOCRINOLOGY(2016)
摘要
Glucagon and alpha-cell dysfunction are critical in the development of hyperglycemia during diabetes both in humans and rodents. We hypothesized that alpha-cell dysfunction leading to dysregulated glucagon secretion in diabetes is due to both a lack of insulin and intrinsic defects. To characterize alpha-cell dysfunction in diabetes, we used glucagon-Venus transgenic male mice and induced insulinopenic hyperglycemia by streptozotocin administration leading to alterations of glucagon secretion. We investigated the in vivo impact of insulinopenic hyperglycemia on glucagon-producing cells using FACS-sorted alpha-cells from control and diabetic mice. We demonstrate that increased-glucagonemia in diabetic mice is mainly due to increases of glucagon release and biosynthesis per cell compared with controls without changes in alpha-cell mass. We identified genes coding for proteins involved in glucagon biosynthesis and secretion, alpha-cell differentiation, and potential stress markers such as the glucagon, Arx, MafB, cMaf, Brain4, Foxa1, Foxa3, HNF4 alpha, TCF7L2, Glut1, Sglt2, Cav2.1, Cav2.2, Nav1.7, Kir6.2/Sur1, Pten, IR, NeuroD1, GPR40, and Sumo1 genes, which were abnormally regulated in diabetic mice. Importantly, insulin treatment partially corrected alpha-cell function and expression of genes coding for proglucagon, or involved in glucagon secretion, glucose transport and insulin signaling but not those coding for cMAF, FOXA1, and alpha-cell differentiation markers as well as GPR40, NEUROD1, CAV2.1, and SUMO1. Our results indicate that insulinopenic diabetes induce marked alpha-cell dysfunction and molecular alteration, which are only partially corrected by in vivo insulin treatment.
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关键词
male insulinopenic diabetic mice,diabetic mice
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