Study of Antioxidants in Combination with Choline Chloride in Mouse Model of Allergic Airway Disease

Inflammation is accompanied by oxidative stress in respiratory allergic disorders. In present study, combination of choline chloride (ChCl) and antioxidants was used as therapy in mouse model of allergic airway disease. Balb/c mice were sensitized on days 0, 7th, 14th and challenged on 25-30 days with cockroach extract (CE). Treatment was given intranasally on days 31st, 33rd, 35th, 37th and 39th with ChCl, Vitamin C and Se alone and in combinations. AHR was recorded on day 40th, and mice sacrificed for collection of blood, BALF, lungs and spleen to study inflammatory and oxidative stress parameters. Mice immunized with CE showed considerable increase in AHR, Immunoglobulins, Th2 cytokines and intracellular reactive oxygen species (iROS) as compared to PBS control mice. A decrease in iROS, IgE and inflammation score in groups treated with ChCl (31%, 55%, 51%), Vitamin C (34%, 61%, 55%), Se (25%, 52%, 58%), ChCl+VitaminC (40%, 69%, 61%), ChCl+Se (32%, 69%, 53%) and VitaminC+Se (33%, 78%, 53%) was observed. AHR was similar to PBS control in group treated with combination of ChCl, Vitamin C and Se. It also showed reduction in TLC (p<0.01), IgE (p<0.01), Th-2 cytokines IL-4 (p<0.01) and IL-5 (p<0.01), NFkB (p<0.01) and oxidative stress marker 8-isoprostanes (p<0.01) significantly. A combination of ChCl, Vitamin C and Se showed highest therapeutic potential in mice model of allergic airway disease.