Pretreatment optimization of Sorghum pioneer biomass for bioethanol production and its scale-up

•Wide range of acid pre-treatments were studied for sorghum hydrolysis.•Influence of combination of temperatures acid concentrations optimized.•Statistical model was successfully developed for enhanced biomass hydrolysis.•Selected yeast use both hexose and pentose for ethanol production.•Even scale up at 25L resulted in 96% of ethanol conversion efficiency.