An efficient procedure for the expression and purification of HIV-1 protease from inclusion bodies

Hongloan Thi Nguyen,Thuy Thi Nguyen,Quy Thi Vu, Hang Thi Le,Yen Pham, Phuong Le Trinh, Thuan Phuong Bui,Tuannghia Phan

Protein Expression and Purification（2015）

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摘要

•An overexpression of HIV-1 protease encoding gene in Escherichia coli using pET32a(+) system.•A simple purification procedure for the HIV-1 protease using a tandem Q-and Ni-Sepharose columns.•A good refolding of the HIV-1 protease from the denatured recombinant form.

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关键词

HIV-1 protease,Purification,pET32a(+),Inclusion bodies

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