Antinociceptive Activity And Acute Toxicological Study Of A Novel Sulfated Polysaccharide From Caulerpa Cupressoides Var. Lycopodium (Chlorophyta) In Swiss Mice

Caulerpa cupressoides var. lycopodium (Chlorophyta) contains three sulfated polysaccharides (SPs) fractions (SP1, SP2 and SP3); but, their pharmacological properties have been limited. We investigated the antinociceptive activity of non-anticoagulant fraction (SP1) and then its acute toxicological study in male Swiss mice was performed. Animals (19-25 g) received i.v. SP1 30 min. prior to injection 0.8% acetic acid (10 mL kg(-1), i.p.); 1% formalin (20 mu L, i.pl.) or were subjected to thermal stimuli. Open-field test was also performed. Mice were treated i.p. with SP1 or 0.9% saline (0.1 mL 10 g(-1)) for 72h. On the 4th day, the animals were anesthetized and sacrificed in order to collect blood and organs. SP1 (3, 9 or 27 mg kg(-1)) reduced (p < 0.05) the number of writhes induced by acetic acid by 44.21, 47.72 and 90.87%, respectively. SP1 inhibited (p < 0.05) the second phase of the formalin test, without antinociceptive effect in the hot-plate test, suggesting that its analgesic action occurs through of peripheral mechanisms. SP1 did not modify the locomotor activity. SP1 (27 mg kg(-1)) did not cause hepatic or renal dysfunctions, but affected the spleen of animals (p < 0.05). Therefore, SP1 has analgesic action with high tolerance by the animals, presenting its potential applicability in pain conditions.