291 PHYSIOLOGICAL EFFECTS OF HYDROGEN SULFIDE ON LOWER URINARY TRACT FUNCTION IN RATS

You have accessJournal of UrologyBladder and Urethra: Anatomy, Physiology and Pharmacology (I)1 Apr 2013291 PHYSIOLOGICAL EFFECTS OF HYDROGEN SULFIDE ON LOWER URINARY TRACT FUNCTION IN RATS Yusuke Koike, Akira Furuta, Takehito Naruoka, Nozomu Furuta, Yasuyuki Suzuki, Shin Egawa, and Naoki Yoshimura Yusuke KoikeYusuke Koike Tokyo, Japan More articles by this author , Akira FurutaAkira Furuta Tokyo, Japan More articles by this author , Takehito NaruokaTakehito Naruoka Tokyo, Japan More articles by this author , Nozomu FurutaNozomu Furuta Tokyo, Japan More articles by this author , Yasuyuki SuzukiYasuyuki Suzuki Tokyo, Japan More articles by this author , Shin EgawaShin Egawa Tokyo, Japan More articles by this author , and Naoki YoshimuraNaoki Yoshimura Pittsburgh, PA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1675AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Hydrogen sulfide (H2S) is a well-known gas characterized by a peculiar rotten egg smell, causing 85% mortality at a high concentration. Although the toxic effects produced by high concentrations of H2S have widely been investigated, its biological activity at physiological concentration remains unclear, despite that H2S is endogenously generated via non-enzymatic and enzymatic pathways in mammals. H2S is now regarded as the third endogenous gaseous transmitter besides nitric oxide (NO) and carbon monoxide (CO). The smooth muscle relaxant activity of H2S has recently been noted in the cardiovascular system, gastrointestinal and reproductive organs although there are few reports concerning the roles of H2S in the lower urinary tract. Thus, we examined the physiological effects of H2S on bladder and urethral function in rats. METHODS Under isoflurane anesthesia, polyethylene catheters were implanted into the bladder dome and internal jugular vein in Sprague-Dawley female rats. The animals were then placed into a sling-suit harness and allowed to recover from anesthesia, so that the experiments were performed in an awake condition. Cystometry and electromyography of the external urethral sphincter (EUS-EMG) were simultaneously recorded during intravesical infusion (0.04ml/min) of saline (control) or sodium hydrogen sulfide (NaHS; 0.01, 0.03, 0.1, 0.3mg/kg), a donor of H2S, applied at 30 min intervals. In other animals in which the pelvic and hypogastric nerves were bilaterally transected to eliminate voiding reflex, leak point pressures (LPPs) were measured until fluid leakage occurred from the urethral orifice during 3ml/min saline infusion into the bladder. Mean blood pressures were also monitored. RESULTS LPPs were significantly decreased by the application of 0.03mg/kg or higher concentrations of NaHS compared with controls. However, there were no significant changes in cystometric parameters, EUS-EMG activities or mean blood pressures during the cumulative administration of NaHS. CONCLUSIONS These results suggest that H2S has a direct relaxant effect on urethral smooth muscle in the lower urinary tract to reduce urethral outlet resistance and that activities of bladder smooth muscle, urethral striated muscle and vascular smooth muscle were not altered by the systemic application of H2S donor at physiological concentrations. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e118-e119 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Yusuke Koike Tokyo, Japan More articles by this author Akira Furuta Tokyo, Japan More articles by this author Takehito Naruoka Tokyo, Japan More articles by this author Nozomu Furuta Tokyo, Japan More articles by this author Yasuyuki Suzuki Tokyo, Japan More articles by this author Shin Egawa Tokyo, Japan More articles by this author Naoki Yoshimura Pittsburgh, PA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...