MP28-18 TH1 IMMUNOREACTION INFLUENCE ON SURVIVAL IN MUSCLE INVASIVE BLADDER CANCER: PROOF OF CONCEPT

You have accessJournal of UrologyBladder Cancer: Basic Research II1 Apr 2014MP28-18 TH1 IMMUNOREACTION INFLUENCE ON SURVIVAL IN MUSCLE INVASIVE BLADDER CANCER: PROOF OF CONCEPT Alexandre Ingels, Rafael Sanchez-Salas, Nina Weber, Pierre Validire, Dominique Prapotnich, Fabien Olivier, Kaixin Yu, Christian Doudeau, Stephane David, Rodolphe Merrina, Marc Galiano, Francois Rozet, Eric Barret, Geraldine Pignot, Jean-Jacques Patard, and Xavier Cathelineau Alexandre IngelsAlexandre Ingels More articles by this author , Rafael Sanchez-SalasRafael Sanchez-Salas More articles by this author , Nina WeberNina Weber More articles by this author , Pierre ValidirePierre Validire More articles by this author , Dominique PrapotnichDominique Prapotnich More articles by this author , Fabien OlivierFabien Olivier More articles by this author , Kaixin YuKaixin Yu More articles by this author , Christian DoudeauChristian Doudeau More articles by this author , Stephane DavidStephane David More articles by this author , Rodolphe MerrinaRodolphe Merrina More articles by this author , Marc GalianoMarc Galiano More articles by this author , Francois RozetFrancois Rozet More articles by this author , Eric BarretEric Barret More articles by this author , Geraldine PignotGeraldine Pignot More articles by this author , Jean-Jacques PatardJean-Jacques Patard More articles by this author , and Xavier CathelineauXavier Cathelineau More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.667AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Immunoscore concept seems particularly relevant in bladder carcinoma since immune response role in this disease is clearly known. Objective: proof-of-concept study to define immunoscore in bladder cancer studying Th1immuno-reaction. METHODS From 10/2005 to 04/2010 252 patients underwent primary TURB-T at our Institution. Retrospective review of a 30 patients selected cohort with pT1 and Muscle Invasive Bladder Cancer (MIBC) lesions was performed. Clinico-pathological features and follow-up were collected. Pathology blocks were marked with CD3 (2GV6, Ventana) and CD8 (C8/144, Dako). Immune cells density in stroma reaction (SR) was measured on 5 high power field (HPF) by a dedicated uro-pathologist blinded for patient's evolution. Statistics: Pearson coefficient to test correlation, Kolmogorov-Smirnov and Shapiro-Wilk tests for normal repartition of data. Student test or non-parametric Wilcoxon test as appropriate to compare mean between 2 groups. Log-rank test to compare survival probability. Univariate Cox model to assess survival's predictors. RESULTS Clinico-pathological characteristics are presented in Table 1. 10 pT1 cases and 20 MIBC cases were analyzed. Immunohistological analysis for pT1 lesions featured limited SR. For MIBC, mean density of lymphocytes in the SR was of 105/HPF (CD3) and 86/HPF (CD8). Lymphocytes T3 and T8's mean densities were strongly correlated to each other (p=0.0001). Survivors harbored higher lymphocytes densities vs non survivors (T3: p=0.0319; T8: p=0.0279). Patients harboring low T3 (p=0.0294) and T8 (p=0.0294) tumor's densities had significant higher mortality on survival analysis. T3 (p=0.026) and T8 (p=0.020) lymphocytes densities were independently associated with cancer-specific survival. CONCLUSIONS High T3 and T8 lymphocytes stroma reaction densities are associated with better cancer specific survival for =>pT2 lesions. Th1 immune reaction to the tumor seems to be protective for bladder cancer. Further evaluation is warranted. Fig 1. Survival curve high (blue) vs low (red) LT8 densities. Threshold : 86/HPF (mean) Fig 2. Staining of LT8 infiltration into tumor margin invasion; HPF (*400) © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e303 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Alexandre Ingels More articles by this author Rafael Sanchez-Salas More articles by this author Nina Weber More articles by this author Pierre Validire More articles by this author Dominique Prapotnich More articles by this author Fabien Olivier More articles by this author Kaixin Yu More articles by this author Christian Doudeau More articles by this author Stephane David More articles by this author Rodolphe Merrina More articles by this author Marc Galiano More articles by this author Francois Rozet More articles by this author Eric Barret More articles by this author Geraldine Pignot More articles by this author Jean-Jacques Patard More articles by this author Xavier Cathelineau More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...