MP79-16 HEAT SHOCK PROTEIN 90 IS A POTENTIAL NEW BIOMARKER OF SEVERE ACUTE REJECTION AFTER RENAL TRANSPLANTATION

You have accessJournal of UrologyTransplantation & Vascular Surgery: Renal Transplantation & Vascular Surgery I1 Apr 2015MP79-16 HEAT SHOCK PROTEIN 90 IS A POTENTIAL NEW BIOMARKER OF SEVERE ACUTE REJECTION AFTER RENAL TRANSPLANTATION Takeshi Maehana, Toshiaki Tanaka, Hiroshi Kitamura, Nobuyuki Fukuzawa, Satoshi Takahashi, Hideki Ishida, Hiroshi Harada, Kazunari Tanabe, and Naoya Masumori Takeshi MaehanaTakeshi Maehana More articles by this author , Toshiaki TanakaToshiaki Tanaka More articles by this author , Hiroshi KitamuraHiroshi Kitamura More articles by this author , Nobuyuki FukuzawaNobuyuki Fukuzawa More articles by this author , Satoshi TakahashiSatoshi Takahashi More articles by this author , Hideki IshidaHideki Ishida More articles by this author , Hiroshi HaradaHiroshi Harada More articles by this author , Kazunari TanabeKazunari Tanabe More articles by this author , and Naoya MasumoriNaoya Masumori More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.2873AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Heat shock protein 90 (HSP90), a molecular chaperone associated with the activation of client proteins, was recently reported to play an important role in immunologic reactions. To date, the role of HSP90 in solid organ transplantations has remained unknown. The purpose of this study was to examine the involvement of HSP90 in acute rejection (AR) of renal transplantation. METHODS Using ELISA, we examined the serum HSP90 (sHSP90) concentrations of 133 samples from 32 kidney allograft recipients, including those who developed acute antibody-mediated rejection (AABMR), acute T cell-mediated rejection (ATMR), chronic antibody-mediated rejection (CABMR), calcineurin inhibitor-related nephropathy (CNIN) and BK virus nephropathy (BKVN). In addition, we measured HSP90 concentrations in the supernatant of human aortic endothelial cells (HAEC) cocultured with a cell-specific anti-HLA antibody and complement (in vitro AABMR model). Furthermore, sHSP90 was examined in mice receiving skin allotransplantation (STx) before and after sensitization. RESULTS sHSP90 levels at the time of ATMR and AABMR were significantly higher (median 26.71 ng/ml and 31.64 ng/ml, respectively) than those when there was no evidence of rejection (median 6.85 ng/ml, p < 0.001). Recipients with vasculitis tended to have a higher sHSP90 level than those having tubulitis alone. On the other hand, the levels in patients with CABMR, CNIN and BKNV were not increased (Figure). Moreover, in most cases with AR, sHSP90 decreased after the treatment. The HSP90 concentration in the supernatant of HAEC cocultured with anti-HLA IgG was significantly higher than in that with nonspecific IgG (3.11 ± 1.02 ng/ml vs. 0.61 ± 0.02 ng/ml, p = 0.03). In mice with STx, sHSP90 was elevated at the time of rejection of the first graft (presensitization, median 41.37 ng/ml) compared with that before transplantation (13.88 ng/ml, p = 0.002), and showed a further increase at the time of rejection of the second graft (postsensitization, 57.21 ng/ml). CONCLUSIONS The results suggest that HSP90 plays a role in the development of AR, especially with vasculitis, in organ transplantation and that it is potentially a new biomarker to detect severe AR in kidney recipients. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e1016-e1017 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Takeshi Maehana More articles by this author Toshiaki Tanaka More articles by this author Hiroshi Kitamura More articles by this author Nobuyuki Fukuzawa More articles by this author Satoshi Takahashi More articles by this author Hideki Ishida More articles by this author Hiroshi Harada More articles by this author Kazunari Tanabe More articles by this author Naoya Masumori More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...