396 TUMORIGENIC AND PROGNOSTIC SIGNIFICANCE OF RASSF1A EXPRESSION IN NON-MUSCLE-INVASIVE LOW-GRADE (WHO G1 AND G2) BLADDER CANCER

You have accessJournal of UrologyUrothelial Cancer: Natural History & Pathophysiology/Marker1 Apr 2012396 TUMORIGENIC AND PROGNOSTIC SIGNIFICANCE OF RASSF1A EXPRESSION IN NON-MUSCLE-INVASIVE LOW-GRADE (WHO G1 AND G2) BLADDER CANCER Yun-Sok HA, Yong-June KIM, Jeong Hyun KIM, Sang-Cheol Lee, Isaac Y.I. KIM, Wun-Jae Kim, Jeong Won Seo, Won-Tae Kim, Yun-Byung Chae, and Seok Joong Yun Yun-Sok HAYun-Sok HA New Brunswick, NJ More articles by this author , Yong-June KIMYong-June KIM Cheongju, Korea, Republic of More articles by this author , Jeong Hyun KIMJeong Hyun KIM Chun cheon, Korea, Republic of More articles by this author , Sang-Cheol LeeSang-Cheol Lee Cheongju, Korea, Republic of More articles by this author , Isaac Y.I. KIMIsaac Y.I. KIM New Brunswick, NJ More articles by this author , Wun-Jae KimWun-Jae Kim Cheongju, Korea, Republic of More articles by this author , Jeong Won SeoJeong Won Seo Daejeon, Korea, Republic of More articles by this author , Won-Tae KimWon-Tae Kim Cheongju, Korea, Republic of More articles by this author , Yun-Byung ChaeYun-Byung Chae Cheongju, Korea, Republic of More articles by this author , and Seok Joong YunSeok Joong Yun Cheongju, Korea, Republic of More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.460AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES To assess RASSF1A expression in low-grade, non-muscle-invasive bladder cancer (NMIBC) in an attempt to understand RASSF1A function. METHODS 101 tumor tissues and normal donor-matched adjacent tissues from patients with primary low-grade NMIBC were selected. RASSF1A expression was measured by quantitative real-time PCR (qPCR) and expressed as the ratio between the normal tissue level and tumor tissue level (N/T ratio). RASSF1A promoter methylation was assessed using methylation-specific polymerase chain reaction (MS-PCR). RESULTS RASSF1A mRNA expression was significantly lower in patients with RASSF1A methylation than in those with no methylation (P = 0.046), and RASSF1A mRNA expression was lower in tumor tissues than in matched normal bladder mucosae (P< 0.001). The RASSF1A N/T ratio was higher in G2 and large tumors than G1 and small tumors (P< 0.05 for each). Kaplan-Meier estimates revealed that the differences in the RASSF1A N/T ratio were associated with significant differences in time to tumor recurrence (P = 0.018) in low-grade NMIBC. Multivariate Cox regression analysis showed that the RASSF1A N/T ratio [hazards ratio (HR): 2.354, P= 0.015] was an independent predictor of recurrence. CONCLUSIONS Decreased RASSF1A expression correlates with the transition from normal mucosa to bladder cancer and has prognostic value in low-grade NMIBC. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e162 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Yun-Sok HA New Brunswick, NJ More articles by this author Yong-June KIM Cheongju, Korea, Republic of More articles by this author Jeong Hyun KIM Chun cheon, Korea, Republic of More articles by this author Sang-Cheol Lee Cheongju, Korea, Republic of More articles by this author Isaac Y.I. KIM New Brunswick, NJ More articles by this author Wun-Jae Kim Cheongju, Korea, Republic of More articles by this author Jeong Won Seo Daejeon, Korea, Republic of More articles by this author Won-Tae Kim Cheongju, Korea, Republic of More articles by this author Yun-Byung Chae Cheongju, Korea, Republic of More articles by this author Seok Joong Yun Cheongju, Korea, Republic of More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...