PCR Product Detector with LED-Photodiode Fluorescence Sensing in a Nanoliter Flow-Cell for the High-Throughput Detection of Double-Stranded DNA
Procedia Engineering(2011)
Abstract
We present an endpoint PCR product sensing system for online detection of double-stranded DNA (dsDNA) down to 1 ng/mu l in a flow-through design with a throughput of up to 144 samples per hour. The device is assembled from off-the-shelf microfluidic and optical components with a chamber volume of 520 nl without any lenses. Fluorescence sensing is realized with a LED-Photodiode combination, which makes this system practicable for every lab. The direct combination with a continuous-flow PCR system realises an integrated diagnostic device for pathogen detection (C) 2011 Published by Elsevier Ltd.
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Key words
PCR,LED,photodiode,DNA,detection,sensor,SYBR Green,lens-free,flow cell,high throughput
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