Development of a genomic engineering tool in Saccharomyces cerevisiae

•Circumvent the plasmid-incurred problems by the genomic engineering tool.•Metabolic engineering of yeast by insertion of heterologous genes and controlled expression of genomic genes.•Cycling improvement of yeast by repeated use of the tool based on the Cre/loxP recombination system.•Reduce the unintentional rearrangement event using the mutant loxP sites.