NMR structural characterization of the N-terminal active domain of the gyrase B subunit from Pseudomonas aeruginosa and its complex with an inhibitor

•The bis-pyridylurea inhibitor improved NMR spectra of pGyrB.•The inhibitor binds to pGyrB with a binding constant of 54.6nM.•Backbone assignments of both free and inhibitor bound pGyrB were obtained.•The Inhibitor did not alter pGyrB structure.•The inhibitor binds to ATP binding pocket.