Structural insights into the loss of catalytic competence in pectate lyase activity at low pH

Salyha Ali,Chresten R. Søndergaard,Susana Teixeira,Richard W. Pickersgill

FEBS Letters（2015）

引用 7|浏览6

暂无评分

摘要

•Two catalytic calcium-ions facilitate proton abstraction in pectate lyase.•At low pH aspartates become protonated and the catalytic calcium-ions are not bound.•The Michaelis complex is not formed in the absence of the catalytic calcium-ions.

查看译文

关键词

Pectate lyase,pH dependence of activity,Michaelis complex,Loss of calcium-binding,Catalysis

AI 理解论文

溯源树

样例

生成溯源树，研究论文发展脉络

Chat Paper

正在生成论文摘要