202 Hydrogen Sulfide (H 2 S) Inhibits Bile Acid Receptor TGR5-Mediated GLP-1 Release From Enteroendrocrine Cells: Possible Involvement of H 2 S in Altered Glucose Metabolism in Diabetes

G A A b st ra ct s gene expression was lower in the Ift88ΔS mice. Despite the rapid induction of gastrin secretion, Ift88ΔS mice showed delayed induction of acid secretion with food intake, and lower mRNA levels of both H+K+ATPase α and β-subunits. In these mice, gastrin, ghrelin, and somatostatin gene expression did not change in response to food intake as in controls. At age 5 months, Ift88ΔS stomachs were histologically normal. However when analyzed at 8 months, mice developed gastric pathology associated with endocrine dysfunction including foveolar hyperplasia, hypergastrinemia, and hypochlorhydria. Epithelial deletion of Kif3a, another gene required for cilia formation, recapitulated the phenotypes observed in Ift88ΔS mice. Conclusion: Overall, our results demonstrated that gastric epithelial cilia regulate endocrine cell function and gastric homeostasis by sensing luminal food.