Analysis of survivin, XIAP and FoxM1 as potential chemoresistance factors in breast cancer cells

Materials and methods Human breast carcinoma cell lines MCF7 (wild-type p53) and MDA-MB-231 (mutant p53) were exposed to dox and citotoxicity was assessed through the MTT assay. Apoptosis was detected through analysis of flow cytometry DNA content and caspases-3, -7 and -9 levels. Survivin, XIAP, p53 and FoxM1 levels were assessed by Western blotting and Survivin and XIAP mRNA levels, by Real Time PCR. Transfections with the Survivin-encoding plasmid and siRNA against Survivin and XIAP were performed using Lipofectamine reagent.