Optimization of jasmonates bioproduction

Several studies have shown that jasmonates (jasmonic acid, (+)-7-isojasmonic acid and methyl jasmonate) exhibit potential anticancer activity, with the advantage of displaying selective cytotoxicity to cancer cells that spares normal lymphocytes to this function [1]. This class of compounds is present in several families of plants, being also produced by certain microorganisms, including Gibberela fujikuroi and Botryosphaeria rhodina. Bioprocess studies based on the use of strains of B. rhodina have shown the potential of this microorganism to produce jasmonates under controlled conditions [2]. There are several factors that affect the productivity of a fermentative process, including the strain of the microorganism, the inoculum, and the morphological and rheological properties of the broth [3]. Once selected the most producing strains, it becomes necessary to define the conditions of the fermentation process, and the standardization of the inoculum is of fundamental importance when seeking increased productivity. In view of the morphology of filamentous fungus B. rhodina and the difficulty to induce sporulation, the standardization of the inoculum is a major challenge. In this context, the aim of this work was to conduct the standardization of B. rhodina inoculum preparation for jasmonate bioproduction.