Isolation, identification and screening of hidrolytic enzymes producing phylloplane yeasts

Background Microorganisms are common colonizers of superficial (phylloplane) and internal tissues (endophyte) of a variety plant species [1]. Phylloplane yeasts are considered a promising source of new and interesting biotechnological activities, particularly hydrolytic enzymes; however, the diversity of yeasts colonizing plant phylloplane and is still poorly known for most plant species. Considering the immense diversity of Brazilian flora, which include many species with medicinal properties, this knowledge is even more limited. Given the above, this study objective to isolate, identify and to evaluate the production of hidrolytic enzymes of yeasts associated with leaves, stems and flowers of alecrim-do-campo (Baccharis dracunculifolia DC), a plant recognized as the main source of exudates used by bees to produce green propolis and also, for its medicinal properties.