Graphene oxide based fluorescence resonance energy transfer and loop-mediated isothermal amplification for white spot syndrome virus detection

•We developed a white spot syndrome virus (WSSV) DNA detection method based on loop-mediated isothermal amplification (LAMP) combined with fluorescence resonance energy transfer (FRET) between graphene oxide (GO) and fluorescein isothiocyanate-labeled probe (FITC-probe).•This technique has a detection limit 10 copies of WSSV plasmid DNA or 0.6fg of DNA extracted from shrimp infected with WSSV and no cross contamination.•GO-FRET with LAMP technique is promising for fast, sensitive and specific DNA detection and could be apply to point-of-operating system.