Engineering a thermostable iron superoxide dismutase based on manganese superoxide dismutase from Thermus thermophilus

Hui Li,Zhimei Feng,Yajie Sun,Shoujiao Ning,Wanlong Zhou,Ao Liu,Fang Pan,Xiubo Zhao,Hu Zhu,Jianr Lu

Process Biochemistry（2016）

Cited 4|Views2

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Abstract

The site-directed mutations of a thermostable superoxide dismutase (SOD) from Thermus thermophilus HB27 can change the metal binding specificity of SOD from Mn to Fe. Furthermore, both mutants can exhibit much higher activity and stability than the wild-type Mn-SOD.

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Key words

Manganese superoxide dismutase,Site-directed mutagenesis,Multiple sequence alignment,Megaprimer-PCR,Metal-binding specificity,Thermostable Fe-SOD

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