Optimization of in vivo crosslinking technique for the study of AlpB-protein interactions in Lysobacter sp. XL1 cells

L A Krasovskaya,Natalya V Rudenko,Olesia P Shuvalova,Natalya A Sukharicheva,S G Abbasova,Nikolai P Skiba,O A Stepnaya

Process Biochemistry（2013）

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Abstract

•DSP-mediated crosslinking in bacterial cells of Lysobacter sp. XL1.•Selection of DSP cross-linker optimal concentration.•Determination of optimal crosslinking reaction time.•Detection of DSP-linked protein complexes by monoclonal Ab against the AlpB enzyme.•Preparation of AlpB-containing protein complexes in soluble form.

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Key words

Crosslinking,Dithiobis(succinimidylpropionate) (DSP),Protein–protein interactions,Lysobacter,Exoenzyme,Secretion

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