No Selection For Env With Shorter Variable Loops In Acute Hiv-1 Infection

AIDS Research and Human RetrovirusesVol. 30, No. S1 Transmission and Viral DiversityFree AccessNo Selection for Env with Shorter Variable Loops in Acute HIV-1 InfectionMorgane Rolland, Sodsai Tovanabutra, Eric Sanders-Buell, Meera Bose, Anne Marie O Sullivan, Shana Howell, Kultida Poltavee, Jenica Lee, Grace Ibitamuno, Sevan Muhammad, Bahar Ahani, Steven Lepore, Elizabeth Harbolick, Celina Oropeza, Joey Patterson, Adam Bates, Michelle Lazzaro, Gustavo Kijak, Kalpana Dommaraju, Christopher Herr, Leigh Anne Eller, Sorachai Nitayaphan, Kathleen Rono, Lucas Maganga, Arthur Sekiziyivu, Nelson Michael, Jerome Kim, and Merlin RobbMorgane RollandMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Sodsai TovanabutraMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Eric Sanders-BuellMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Meera BoseMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Anne Marie O SullivanMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Shana HowellMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Kultida PoltaveeMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Jenica LeeMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Grace IbitamunoMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Sevan MuhammadMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Bahar AhaniMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Steven LeporeMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Elizabeth HarbolickMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Celina OropezaMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Joey PattersonMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Adam BatesMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Michelle LazzaroMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Gustavo KijakMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Kalpana DommarajuMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Christopher HerrMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Leigh Anne EllerMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this author, Sorachai NitayaphanAFRIMS, Bangkok, ThailandSearch for more papers by this author, Kathleen RonoWRP, Kericho, KenyaSearch for more papers by this author, Lucas MagangaMMRP, Mbeya, Tanzania, United Republic ofSearch for more papers by this author, Arthur SekiziyivuMUWRP, Kampala, UgandaSearch for more papers by this author, Nelson MichaelMHRP, Silver Spring, MD, United StatesSearch for more papers by this author, Jerome KimMHRP, Silver Spring, MD, United StatesSearch for more papers by this author, and Merlin RobbMHRP;HJF, Silver Spring, MD, United StatesSearch for more papers by this authorPublished Online:30 Oct 2014https://doi.org/10.1089/aid.2014.5396.abstractAboutSectionsPDF/EPUB Permissions & CitationsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail P25.11Background: Previous reports showed that HIV-1 viruses from early infection had shorter HIV-1 Env variable loops and could be more sensitive to neutralization. However, findings on HIV-1 subtype C and A were not confirmed with subtype B and there were uncertainties as to whether loop lengths evolved in the first weeks of infection. Here we characterized Env variable loops (V1-V5) in subjects newly infected (diagnosis at a median of 4 days after the last negative visit) with different HIV-1 subtypes.Methods: We sequenced 1,204 HIV-1 strains from 49 subjects enrolled in Kenya, Tanzania, Uganda and Thailand at a median of 4, 33 and 171 days after diagnosis. These sequences were compared to 624 independent sequences from chronic infection.Results: Reflecting the distribution of subtypes in Thailand and East Africa, our cohort included individuals infected with CRF01_AE (n=15), subtype A1 (n=10), C (n=4), and different A1/C/D recombinant strains (n=17). V1 loops varied between 8 and 34 amino acid (AA) (IQR=16-23), while V2 loops varied between 36 and 51 AA (IQR=40-44). Variable loop lengths did not differ between HIV-1 subtypes (p>0.230). Next, we compared loop lengths from our cohort to values from chronically infected subjects: for 280 sequences isolated past 2000, V1 loops varied between 5 and 42 AA (IQR=18-24), and V2 loops between 36 and 74 AA (IQR=40-45). There was no evidence that variable loops from acutely-infected individuals were shorter than those from chronically-infected individuals (p>0.188). Finally, we found that Env variable loop lengths did not increase over the first six months of follow-up in our cohort (p>0.352).Conclusions: Env sequences sampled in the first week of HIV-1 infection showed a wide range of variable loop lengths, making them undistinguishable from sequences from chronic infection. Our findings indicate that viruses with shorter Env variable loops are not selected for in the establishment of HIV-1 infection.FiguresReferencesRelatedDetails Volume 30Issue S1Oct 2014 InformationCopyright 2014, Mary Ann Liebert, Inc.To cite this article:Morgane Rolland, Sodsai Tovanabutra, Eric Sanders-Buell, Meera Bose, Anne Marie O Sullivan, Shana Howell, Kultida Poltavee, Jenica Lee, Grace Ibitamuno, Sevan Muhammad, Bahar Ahani, Steven Lepore, Elizabeth Harbolick, Celina Oropeza, Joey Patterson, Adam Bates, Michelle Lazzaro, Gustavo Kijak, Kalpana Dommaraju, Christopher Herr, Leigh Anne Eller, Sorachai Nitayaphan, Kathleen Rono, Lucas Maganga, Arthur Sekiziyivu, Nelson Michael, Jerome Kim, and Merlin Robb.No Selection for Env with Shorter Variable Loops in Acute HIV-1 Infection.AIDS Research and Human Retroviruses.Oct 2014.A185-A185.http://doi.org/10.1089/aid.2014.5396.abstractPublished in Volume: 30 Issue S1: October 30, 2014PDF download