Lc-Ms/Ms Analysis Of Sugarcane Extracts And Differentiation Of Monosaccharides Moieties Of Flavone C-Glycosides
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES(2013)
摘要
LC-MS/MS data of the flavones from extracts of nonmodified and transgenic sugarcane (Bowman-Birk and Kunitz) led to the proposition of characteristic fragmentations that can be applied to identify the monosaccharides glucose, arabinose, and rhamnose in C-glycosylflavones. Collision-induced dissociation (CID) MS/MS generated diagnostic ions for different linked sugars in C-glycosylflavoneO-glycoside and C-glycosylflavoneO-acid derivatives, and the combination of MS data with postcolumn derivatization using UV shift reagents (which provide complementary information to determine the substitution patterns in the flavone skeleton) allowed for the identification of seven flavones not previously reported in sugarcane: 6-methoxyluteolin-8-C-arabinosyl-7-O-glucoside; diosmetin-8-C-arabinosyl-7-O-arabinoside; vitexin-7-O-rhamnoside; diosmetin-8-C-rhamnosyl-7-O-glucoside; tricetin-8C-(6?-O-sinapoylglucoside); 7,4' di-O-methylapigenin-8-C-arabinosyl-rhamnoside; and luteolin-8-C-rhamnosyl-7-O-rhamnoside.
更多查看译文
关键词
CID-MS, MS, flavonoids, LC-UV-MS, postcolumn derivatization, sugarcane, transgenic plants
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要