Batch Equilibrium And Kinetic Studies Of Plasmid Pci Adsorption Onto Perfusion Particles
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES(2015)
Abstract
The demand for efficient production methods of plasmid DNA (pDNA) has increased vastly in response to rapid advances in the use of pDNA for third-generation vaccines. The process development requires conduct basic studies in batch format to determine the characteristic parameters of the biomolecule-adsorbent system. In this work, the equilibrium and kinetic performance of the plasmid pCI adsorption onto ion-exchange perfusive particles POROS 50 HQ was studied. The isotherm obtained was appropriately described by the Langmuir model, with the parameters q (m)=29.15mg PCI/mL of adsorbent and K (d)=0.0145mg de pCI/mL. These values show a greater capacity and more specific adsorption of pCI onto the anion-exchange (AE) perfusive particles then onto AE adsorptive membranes previously studied. The kinetics of adsorption was described adequately by a lumped resistance model using a kinetic constant of k (1)=0.0067mL/mg s.
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Key words
plasmid purification, DNA vaccines, adsorption isotherms, adsorption models, batch adsorption kinetics, perfusion adsorption
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