Comparison of two culture methods for the production of haploids by anther culture in Solanum chacoense

The response of anther culture to two culture procedures was tested using anthers of a clone of self-incompatible Solanum chacoense Bitt. grown under field conditions and in a greenhouse. The use of a high level of sucrose (20%) in the induction medium increased the proportion of anthers producing macroscopic calli. This effect was more pronounced for plants grown under field conditions than for those grown in a greenhouse. The later regeneration process, using plants from either source, was not affected by this procedure.