Genetic and Molecular Mapping of Stripe Rust Resistance Genes in Wheat Cultivar Zhongliang 12

Stripe rust, caused by Puccinia striiformis f.sp. tritici (Pst), is one of the most widespread and destructive diseases of wheat worldwide. Resistance breeding is constantly pursued for decades to tackle the variations of prevalent Pst races. Zhongliang 12 has strong resistance to abiotic stresses, wide adaptability, higher resistance to stripe rust and excellent biological characteristics. To identify the resistance gene(s) against stripe rust, Zhongliang 12 was crossed with stripe rust susceptible genotype Mingxian 169, and F-1, F-2, F-2:3 and BC1 progenies were tested with Chinese Pst race CYR30 and CYR31 in seedling stage in greenhouse. Zhongliang 12 possessed different dominant genes for resistance to each race. Linkage maps were constructed with four simple sequence repeats (SSRs) markers, Xwmc695, Xcfd20, Xbarc121 and Xbarc49, for the gene on wheat chromosome 7AL conferring resistance to CYR30 (temporarily designated as Yrzhong12-1) with genetic distance ranging from 3.1 to 10.8cM and four SSR markers, Xpsp3003, Xcfd2129, Xwmc673 and Xwmc51, for the gene on wheat chromosome 1AL conferring resistance to CYR31 (temporarily designated as Yrzhong12-2) with genetic distance ranging from 3.9cM to 9.3cM. The molecular markers closely linked to each gene should be useful in marker-assisted selection in breeding programmes for against stripe rust.