Hyperaldosteronism And Altered Expression Of Sgk 1 Dependent Sodium Transporter In Type 2 Diabetes Lead To Salt Dependency Of Blood Pressure

This study was designed to test whether altered aldosterone-related sodium handling leads to salt sensitive blood pressure (RR) in diabetes. As a model of type 2 diabetes Zucker Diabetic Fatty (ZDF) rats and as euglycemic controls Zucker lean (ZDL) rats were divided into groups receiving normal (0.28%) and high salt (5.5%) diet (ZDF+S, ZDL+S) for 10 weeks. RR was measured weekly by tail cuff method. MRNA expressions of serum and glucocorticoid induced kinase 1 (SGK1) and ENaC alpha sodium transporter were quantified by RT-qPCR. Vascular hypertrophy (media to lumen ratio, M:L) of mesenteric resistance arteries was assessed using a pressurized myograph. RR was significantly higher in ZDF+S vs. ZDF (146±2 vs 133±3 mmHg; p