Investigating triazine-based modification of hyaluronan using statistical designs

•We used statistical designs to study triazine-based derivatization of hyaluronan.•We observed the triazine-method to be more efficient compared to carbodiimide-method.•We evaluated the effect of each factor on the derivatization using fractional factorial design.•We used response surface methodology to control the derivatization and optimize the reaction condition.•We determined the stability, purity and biocompatibility of derivatized hyaluronan.