Pathogen discovery in AIDS-related lymphoma by high-throughput sequencing

Background Approximately 30% of AIDS-related lymphomas (ARL) are associated with infection by the EBV, and about 4% by the KSHV/HHV-8. It is likely that if other lymphomagenic pathogens exist, these associations would occur in the context of ARL. The advent of high throughput sequencing provides a unique opportunity to address this question. High throughput sequencing, followed by computational subtraction of human sequences was used for enrichment of candidate pathogenic sequences. Methods