Vanadium-binding protein in marine plankton from tropical south atlantic ocean
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY(2014)
摘要
We investigated the role of plankton in the vanadium (V) cycle at Cabo Frio, Rio de Janeiro state, Brazil, a region with high V concentration in the atmospheric particles due to marine aerosol. The concentrations of V in plankton vary from 0.08 to 20.9 mu g g(-1) (zooplankton), 0.1 to 28.4 mu g g(-1) (phytoplankton > 64 mu m) and < 0.0005 to 49.0 mu g g(-1) (small phytoplankton > 20 mu m). The V speciation in biomolecules was performed by the use of two strategies: (i) coupling of size exclusion chromatography (SEC) for the fractionation of species with inductively coupled plasma mass spectrometry (ICP- MS) and (ii) with size exclusion anionic exchange chromatography with UV-Vis detector coupled to inductively coupled plasma mass spectrometry (SEC-AE-UV-Vis-ICP-MS). The results showed a single fraction containing V associated with a biomolecule in the range of 8 to 16 kDa, with isoeletric points above 8. The preliminary analyses using matrix-assisted laser desorption-ionization-time of flight (MALDI-TOF) do not permit to identify such biomolecule, considering the broader size range of the proteins obtained.
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关键词
bioaccumulation,biogeochemistry,chemical ecology,HPLC-ICP-MS,metallothionein
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