1LB Development and validation of robust immunohistochemical assays for phospho-histone-H3 and Eg5 as pharmacodynamic biomarkers to support Eg5 inhibitor (LY2523355) clinical trials in patients with advanced malignancies

Twenty Holstein heifers were used in a 120-day feeding trial to study the feeding value of a liquid protein supplement, composed of 10% urea, 80% molasses, 6% ethyl alcohol, 4% phosphoric acid, and an assortment of trace minerals, upon growth and feed consumption. Pasture clippings supplemented with 2 lb. of ground corn were fed to six of the heifers; whereas, cottonseed hulls plus 2 lb. of cottonseed meal and 1 lb. of corn were fed to a second group of seven heifers, and cottonseed hulls, 3 lb. of an alcohol-urea preparation, and 1 lb. of corn were fed to a third group of seven heifers. The average daily gains and pounds of feed per pound of gain were 1.21 and 16.91, 0.78 and 29.48, and 0.88 and 29.22 lb. for the groups receiving pasture clippings, cottonseed hulls plus cottonseed meal, and cottonseed hulls plus the alcohol-urea preparation and corn, respectively. There was no significant difference between the two groups of heifers receiving cottonseed hulls in gains per day or gain per pound of feed eaten. The alcohol-urea preparation represented over 80% of the total protein equivalent.