Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing
CHEMICAL COMMUNICATIONS(2016)
Abstract
A cleavable azo linker was synthesized and reacted with 5-(6)-carboxytetramethyl rhodamine succinimidyl ester, followed by further reactions with di(N-succinimidyl) carbonate and 5-(3-amino-1-propynyl)-2'-deoxyuridine 5'-triphosphate [dUTP(AP(3))] to obtain the terminal product dUTP-azo linker-TAMRA as a potential reversible terminator for DNA sequencing by synthesis with no need for 3'-OH blocking.
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Key words
nucleotide,cleavable azo linker,dna,fluorescence-labeled
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