Development of a rapid and sensitive one-step reverse transcription-nested polymerase chain reaction in a single tube using the droplet-polymerase chain reaction machine

Akemi Yamaguchi,Kazuyuki Matsuda,Akane Sueki,Chiaki Taira,Masayuki Uehara,Yasunori Saito,Takayuki Honda

Clinica Chimica Acta（2015）

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摘要

•One-step RT-nested PCR performed all reactions in a single tube.•BCR–ABL mRNA could be detected within 40min.•The sensitivity of the one-step RT-nested PCR was 0.001%.•This method may be applicable to wide spectrum of genetic tests.

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关键词

PCR,RT-PCR,droplet-PCR,SNPs,CML,ALL

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