Investigation of a tissue engineered tendon model by PS-OCT
Bios(2010)
摘要
A few native tissues, such as tendon, skin and eye, possess highly organized collagenous matrices. In particular, the
collagen fibers in tendon are organized into a hierarchical and unidirectional format, which gives rise to the high tissuespecific
mechanical properties. This organization has been clearly revealed by a conventional polarized light microscope.
The newly developed polarization-sensitive optical coherence tomography (PS-OCT) technique allows non-invasive
visualization of birefringence images arising from orientated structures in a three dimensional format. Our previous
studies of native tendon and tissue engineered tendon by PS-OCT demonstrate that tissue engineered tendon has a far
less perfect collagen fiber organization than native tendon even under dynamic culture conditions. The purpose of this
study is to use PS-OCT to assess the relationship between the degree of birefringence, collagen concentration and fiber
density in model tendon tissues. The model tissue is constructed from an aligned collagen hydrogel and aligned polyester
nanofibers. The effects of the diameter and density of the nanofibers and the collagen concentration in the model have
been investigated. The alignment of collagen fibrils is induced by application of a high magnetic field during
fibrillogenesis while aligned polyester nanofibers are manufactured using the electrospinning technique. It is found that
the collagen concentration, the density and size of nanofiber bundles are the key parameters to produce birefringence in
OCT images. The perfectly aligned collagen hydrogel with concentration as high as 4 mg/ml does not exhibit a
birefringence image until the hydrogel has been compressed and concentrated. Aligned nanofiber bundles have
demonstrated marginal birefringence in the absence of the collagen matrix. These studies enhance our understanding of
how to control and optimize the parameters in tendon tissue engineering.
更多查看译文
关键词
polarization,three dimensional,magnetism,visualization,matrices,skin,birefringence,polarized light,tissue engineering,nanofibers
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要