Gender differences on oxidative stress markers and complement component C3 plasma values after an oral unsaturated fat load test

Objective: Post-prandial lipaemia (PL), oxidative stress (OS), and complement component C3 (C3) values are related to the atherosclerosis process. The post-prandial response of C3 after an oral fat toad test (OFLT) using unsaturated fat is poorly addressed. The aim of this study was to analyze and compare the post-prandial response of OS markers and C3 values in men and women after an OFLT using unsaturated fat. Methods: The study included a total of 22 healthy subjects with normal lipids and normal blood glucose (11 men and 11 pre-menopausal women). An oral unsaturated fat toad test (OFLT: 50g fat per m2 body surface) was performed using a commercial liquid preparation of tong chain triglycerides (SupracaL). OS markers and C3 were measured using standardized methods at fasting state and every 2 h up to 8 h after the OFLT. Results: Men showed statistically significant higher C3, oxidized glutathione (GSSG), and oxidized-reduced glutathione (GSSG/GSH) ratio values at fasting state compared to that obtained in women. In addition, post-prandial C3 values and GSSG/GSH ratios were significantly higher in men compared to women. The GSSG value and GSSG/GSH ratio significantly decreased in men after the OFLT compared to fasting values. In contrast, the post-prandial OS markers decrease observed in women was not statistically significant. Conclusions: In fasting state, men showed higher statistically significant C3 values and OS markers than women. The post -prandial OS markers (0550 and GSSG/GSH ratio) significantly decrease after the OFLT with unsaturated fat in men compared to women. 2019 Sociedad Espanola de Arteriosclerosis. Published by Elsevier Espana, S.L.U. All rights reserved.