In vitro bioactivity of Bioroot™ RCS, via A4 mouse pulpal stem cells

•Osteoinductive properties of Bioroot™ RCS was compared to Kerr's Pulp Canal Sealer™ using the mouse pulp-derived stem cell line A4, which have an osteo/odontogenic potential in vitro.•Trypan Blue exclusion assay was done for cytotoxicity evaluation, immunocytochemical staining was done for SIBLINGs (BSP, DMP 1, Collagen Type 1), and Von Kossa was done for mineralization evaluation.•Bioroot™ RCS was not found cytotoxic as PCS. It did not recruit the pulpal stem cells toward differentiation but preserve their osteo-odontogenic intrinsic properties.