Effect of protein aggregates on characterization of FcRn binding of Fc-fusion therapeutics
Molecular Immunology(2015)
Abstract
•FcRn binding of therapeutic Fc-fusion protein samples has been evaluated.•Assays were developed based on two novel technologies: AlphaScreen and BLI.•Found positive correlation between the level of aggregates and FcRn binding.•The magnitude of the effect depends on the assay format: important to investigate.•Presence of aggregates in the samples can mask the loss of FcRn binding.
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Key words
BLI,CH2–CH3,ELISA,FcRn,FRET,HMW,HPLC,ka,kdis,MES,PBS,SEC,SPR,UV
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