THU0194 Association between autoantibodies and soluble factors in cerebrospinal fluids from patients with neuropsychiatric systemic lupus erythematosus

Background Neuropsychiatric (NP) symptoms are a frequent concern of connective tissue disease (CTD) patients. Although the increased levels of several soluble factors in cerebrospinal fluids (CSF) and lupus autoantibodies (auto Abs) from NP systemic lupus erythematosus (NPSLE) patients 1,2 have been reported, their association remains unknown. Objectives To determine an association of serum- and CSF-anti-U1RNP, SS-A/Ro (SSA) and N-methyl-D-aspartate glutamate receptor (NR2) Abs with CSF-soluble factors in CTD patients with NP manifestations. Methods Serum- and CSF-anti-U1RNP/SSA Abs were examined by RNA-immunoprecipitation assay reported by our group 2 , and ELISA 3 was used for detection of anti-NR2 Abs. IFN-α, IL-8, IP (interferon inducible protein) -10, MCP (monocyte chemoattractant protein) -1, MIG (monokine induced by IFN-γ), RANTES (regulated upon activation normal T cell expressed and secreted), and fractalkine in the same CSF samples were determined by Procarta® Cytokine Assay kit with Bio-Plex TM . Results Eighty-two patients with CTD and NP manifestations (76 of SLE, 3 of mixed connective tissue disease, and 3 of Sjogren’s syndrome) were enrolled. Anti-U1RNP Abs were found in sera from 48 (59%) and in CSF from 15 (18%) patients. CSF-anti-U1RNP Abs were never recognized in serum-anti-U1RNP Ab-negative patients. Among 48 patients with serum-anti-U1RNP Abs, CSF-IFN-α (13.7 vs. 4.9 pg/mL, p p p p Conclusions CSF-anti-U1RNP and NR2 Abs were associated with different subset of soluble factors in CSF, which may have a pathogenic role in NPSLE. Additionally, anti-U1RNP Abs in serum appeared to be involved in the increased levels of CSF-IFN-α and MCP-1 in NPSLE patients with CSF-anti-U1RNP Abs. References Hanley JG, et al. Ann Rheum Dis, 2011; 70: 1726. Sato T, Fujii T, et al. AR 62: 3730. Omdai R, et al. Eur J Neurol, 2005; 12: 392. Disclosure of Interest None Declared