Preparation and characterisation of cellulose ZnO hybrid film by blending method and its glucose biosensor application

This paper reports an inexpensive, flexible and disposable cellulose ZnO hybrid film (CZHF) and its feasibility for a conductometric glucose biosensor. CZHF is fabricated by simply blending ZnO nanoparticles with cellulose solution prepared by dissolving cotton pulp with lithium chloride/N, N-dimethylacetamide solvent. After curing in isopropyl alcohol and water mixture, the CZHF is obtained. The enzyme glucose oxidase is immobilised into the CZHF by physical adsorption method. The enzyme activity of the glucose biosensor increases as the ZnO weight ratio increases linearly. The CZHF can detect glucose in the range of 1-12 mM.