Определение антидепрессантов амперометрическими моноаминоксидазными биосенсорами на основе графитовых печатных электродов, модифицированных многостенными углеродными нанотрубками

Novel amperometric biosensors based on screen-printed graphite electrodes modified with multi-walled carbon nanotubes (MWCNTs) dispersed in various solvents (chitosan, DMF) and immobilized monoamine oxidase (MAO) were developed for the determination of antidepressants imipramine and afobazole. It is established that afobazole and imipramine possess the properties of MAO inhibitors, which allows these pharmaceuticals to be determined within the concentration range of 0.1 mM – 1 nM by recording the oxidation wave of hydrogen peroxide when dopamine and epinephrine are used as MAO substrates. The best analytical performance was observed in the case of MAO biosensors modified by MWCNTs in chitosan solution, for which the lower limit of detectable concentrations was 3 – 9 nM. The main active components in melipramine and afobazole preparations were quantified using the MAO-based amperometric biosensors with an RSD of 0.08.