A reliable and efficient BioPulverizer method in preparing and grinding nematodes for nucleic acid extraction and molecular identification

Due to their tiny size, soil nematodes and plant-parasitic nematodes are challenging to homoge-nize and collect using traditional mortar and pestle methods. To overcome this, we developed a reliable and efficient nematode preparation and grinding method using the BioPulverizer. The method involves creating a modified sample 'sandwich' with liquid nitrogen-frozen nematode samples placed between two layers of aluminum foil. This 'sandwich' facilitates complete homogenization through cryogenic grinding without any sample loss during transfer. The powerful nematode grinding yields high-quality and high-quantity samples, suitable for nucleic acid release and subsequent molecular identification and other downstream applications.