Mass Spectrometry for the Detection and Identification of Proteins

Abstract Originally published in: Analysing Gene Expression. Edited by Stefan Lorkowski, Paul Cullen. Copyright © 2003 Wiley‐VCH Verlag GmbH & Co. KGaA Weinheim. Print ISBN: 3‐527‐30488‐2 The sections in this article are Introduction How does mass spectrometry work? Ionisation of molecules Detection of ions Appearance of spectra Modes of operation Proteomic mass spectrometry Data analysis tools for mass spectroscopic experiments Proteomics servers and databases An example: Identification of an isolated and unknown protein Is quantification possible with mass spectrometry? Analysis of protein modifications Phosphorylation Glycosylation Access to protein tertiary structure Analysis of intact proteins and non‐covalent protein complexes Affinity mass spectrometry Conclusion