Deteksi Dini Infeksi Virus Nipah dengan Uji Enzyme Linked Immunoassay pada Babi di Indonesia

Nipah is an exotic, zoonotic and contagious viral disease which infect pigs and humans. Using ELISA test, serological survey was conducted to gain the information of the presence of Nipah infection in pig in Indonesia. A total of 1471 pig sera collected from North Sumatera, Riau, North Sulawesi and Java were tested using inactivated Nipah antigen and the results indicated that no antibodies against Nipah virus was detected. In order to validate and to confirm the results recorded, 384 pig sera were send to Australian Animal Health Laboratory (AAHL) and were tested using serum neutralization test. Based on the same ELISA results and standardised ELISA techniques in both laboratories, it was concluded that Nipah ELISA test can be done at Balitvet laboratory and applied in Indonesia. The test is needed as a part of technological transfer, as well as to support the Government policy in order to anticipate the entering of Nipah virus infection. Key words: virus nipah, serology, ELISA test, pigs